Cheek cells space eukaryoticcells (cells the contain a nucleus and also other organelles in ~ enclosed in amembrane) that room easily melted from the mouth lining. It"s therefore easy toobtain them because that observation.

You are watching: Human cheek cell under microscope 400x

Some of the main parts the acell include:

1. Cell membrane (outer border of the cell)

2. Cytoplasm (the fluid within the cell)

3. Nucleus (at the facility of the cell and controls cabinet functions)

4. Organelles (e.g. Mitochondria-Organelles are cell structures withspecific functions)

Using organic stainssuch as methylene blue, it"s possible to clearly observe and differentiate thedifferent parts of a cell. This is since the stain will shade some components ofthe cell and also not others, allowing them come be plainly observed.


Sterile cotton swabMicroscope covering slipsMethylene Blue systems (0.5% to 1%)DropperBlotting paper/Tissue paper

How come Prepare a Wet mount of Cheek Cells

Before starting, it"salways vital to ensure that the working surface ar is clean and also that friend arewearing a pair the clean gloves to protect against contamination.

Cheek cells deserve to beeasily derived by gently scraping the inside of the mouth using a clean,sterile cotton swab.

Once the cells have actually been obtained,the adhering to procedure is provided for cheek cell wet mountain preparation:

place a fall of physiological saline on a clean microscope slide(central part of the slide)smear the noodle swab on come the facility (part include the salinedrop) of the clean on slide for about 4 seconds to gain the cells on to the centerof the slideadd a autumn of methylene blue systems on to the smear and also gentlyplace a cover slip on optimal (to covering the stain and the cells)any excess solution have the right to be removed by touching one next of theslide v a file towel or blotting the on slide on the microscopic lense for observation using 4 x or 10x target to uncover the cellsonce the cells have been found, they deserve to then be viewed at highermagnificatio


* Note - supplied cotton swabsand cotton towel need to be for sure discarded in the trash and not left lied onthe functioning table.

cheek cell stained through methylene blue by Joseph Elsbernd at

Why do we have to Stain the Cells?

The cell has differentparts, and those that deserve to absorb stains or dyes are referred to as chromatic.Having soaked up the stain, these components of the cabinet become more visible underthe microscope and can because of this be easily identified from various other parts ofthe same cell.

Without stains, cells would show up to be virtually transparent,making it daunting to identify its parts.

Methylene blue has actually a stringaffinity for both DNA and RNA. When it comes in call with the two, a darkerstain is produced and also can be regarded under the microscope.

The nucleus at thecentral component of the cheek cell contains DNA. Once a fall of methylene blue isintroduced, the cell nucleus is stained, which makes it was standing out and be clearlyseen under the microscope.

Although the entire cell appears light blue incolor, the nucleus at the main part of the cabinet is much darker, i m sorry allowsit to be identified.


On mounting the wet slide,the complying with will it is in observed:

Large irregularly shaped cell with distinct cell membranes.A distinct nucleus in ~ the central part of every individual cell(dark blue in color).


This is basic and funexperiment that will show children the straightforward structure that a cell and also its majorparts. For simple identification that the parts, the parent or teacher have the right to firstshow the youngsters some samples of the cell in advance.This will assist themidentify various parts v ease.

Once this has actually been achieved, youngsters can moveon come the following stage of finding out the functions of these different parts.

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** discover prepared microscopic lense slides and equipment to appropriately perform microscopic lense experiments.

See also: Epithelial Cells

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